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Co-silencing E1 and its homologs in an extremely late-maturing soybean cultivar confers super-early maturity and adaptation to high-latitude short-season regions

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文献出处
Journal of Integrative Agriculture  2022年02期
论文摘要

Soybean(Glycine max(L.) Merr.), a typical short-day plant, is sensitive to photoperiod, which limits the geographical range for its cultivation. In the flowering pathway regulated by photoperiod, E1, a flowering inhibitor in soybean, plays the dominant role in flowering time regulation. Two E1 homologs, E1-like-a(E1 La) and E1-like-b(E1 Lb), play overlapping or redundant roles in conjunction with E1. In the present study, E1 and E1 La/b were simultaneously silenced via RNA interference(RNAi) in Zigongdongdou(ZGDD), an extremely late-flowering soybean landrace from southern China. As a result, RNAi lines showed a much earlier-flowering phenotype and obvious photoperiod insensitivity compared with wild-type(WT) plants. In RNAi transgenic plants, the expression levels of flowering inhibitor GmFT4 and flowering promoters Gm FT2a/Gm FT5a were significantly down-and up-regulated, respectively. Further, the maturity group(MG) of the RNAi lines was reduced from WT ZGDD’s MG Ⅷ(extremely late-maturity) to MG 000(super-early maturity), which can even grow in the northernmost village of China located at a latitude of 53.5°N. Our study confirms that E1 and E1 La/b can negatively regulate flowering time in soybean. The RNAi lines generated in this study, with early flowering and maturity traits, can serve as valuable materials and a technical foundation for breeding soybeans that are adapted to high-latitude short-season regions.

论文目录
关闭目录
1. Introduction
2. Materials and methods
  2.1. Plant materials and growth conditions
  2.2. E1 cloning and RNAi vector construction
  2.3. Soybean transformation and identification of transgene-positive plants
  2.4. Gene expression analysis
  2.5. Phenotyping and statistical analysis
  2.6. Maturity group classification
3. Results
  3.1. RNAi-mediated down-regulation of E1 and E1La/b expression resulted in flowering promotion
  3.2. RNAi lines exhibited high expression levels of Gm FT2a/5a but low expression levels of Gm FT4
  3.3. Photoperiod sensitivity was significantly decreased in RNAi lines
  3.4. RNAi lines were reclassified as an extremely-early MG
4. Discussion
5. Conclusion
Declaration of competing interest
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